77PD/2018

Project title: Analysis of the interactions between nanoparticles and biomolecules to understand the role of nano-bio-interface in predicting nanoparticle behavior and effects on biological

Project director: Asist. Univ. Dr. Miruna-Silvia Stan

University of Bucharest/Faculty of Biology/Dep. of Biochemistry and Molecular Biology

Tel: 021.318.15.75/int 105

Fax: 021.318.15.75/int 103

E-mail: miruna.stan@bio.unibuc.ro

Funding agency: UEFISCDI

Contract number: 77/2018

Project code: PN-III-P1-1.1-PD-2016-1562

Project acronym:  NANO-BIO-INT

Project duration: 24 months (02.05.2018 – 30.04.2020)

Total funding: 250000 RON

Project members: Asist. Univ. Dr. Miruna-Silvia STAN (postdoctoral researcher)

Prof. Univ. Dr. Marieta COSTACHE (mentor)

Project summary

It is widely accepted that upon the nanoparticles (NPs) entrance to the biological fluid their surface will interact with the medium’s components providing a new biological identity for NPs. The interface between particles and cell surface lipid bilayer comprises a series of dynamic interactions which are mediated by the protein corona and it is of great importance to follow the progressive events triggered during exposure to NPs at different time points to obtain relevant analysis valuable for therapeutic drug delivery or other purposes. Therefore, this work envisages a complex characterization of NPs interaction with the three major classes of biomolecules in order to associate the changes in nano-bio-interface with the specific physico-chemical properties of NPs and to obtain certain relationships very useful for further molecular modeling and simulation studies.

Project objective

The objective of the present project is represented by the complex analysis over time of the interaction between nanoparticles with different characteristics (chemical structures, sizes, surfaces) and biomolecules (proteins, lipids and nucleic acids).

Expected results for this project

The expected results will include a deeper understanding of nanoparticles’ biocorona, of their behavior in biological systems, and effect on molecules’ structures, in order to design further strategies for preventing toxicity induced by nanoparticles with well-established properties.

Results of Phase I – 2018

– poster at international conferences

  • Strugari A., Stan M.S. Nanoparticle intestinal transport characterization using in vitro co-culture models. Proceedings of 1st International Online-Conference on Nanomaterials, DOI 10.3390/IOCN_2018-1-05480.

– research article published in ISI journal

  • Stan MS, Badea MA, Pircalabioru GG, Chifiriuc MC, Diamandescu L, Dumitrescu I, Trica B, Lambert C, Dinischiotu A. Designing cotton fibers impregnated with photocatalytic graphene oxide/Fe, N-doped TiO2 particles as prospective industrial self-cleaning and biocompatible textiles. Mat Sci Eng. C 2018, 94:318-332. Impact Factor: 5.080 (2017), Relative influence score: 1.105 (2017). DOI: 10.1177/1528083718779447

– book chapter

  • Stan MS, Strugari AFG, Balas M, Nica IC. Biomedical applications of carbon nanotubes with improved properties. In: Fullerenes, Graphenes and Nanotubes: A Pharmaceutical Approach (ed. Grumezescu AM), Elsevier, Oxford, UK, 2018, p. 31-65. Print ISBN: 978-0-12-813691-1.

– review paper

  • Nica IC., Stan M.S., Dinischiotu A. Current photocatalytic applications of nano-scaled titanium dioxide in the new era of “smart” technologies. Rev. Biol. Biomed. Sci. 2018; 1(2): 43-53. DOI: 10.31178/rbbs.2018.1.2.1.

Scientific and Technical Report

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