Project title: Analysis of the interactions between nanoparticles and biomolecules to understand the role of nano-bio-interface in predicting nanoparticle behavior and effects on biological
Project director: Lect. Univ. Dr. Miruna-Silvia Stan
University of Bucharest/Faculty of Biology/Dept. of Biochemistry and Molecular Biology
Tel: 021.318.15.75/int 105
Fax: 021.318.15.75/int 103
Funding agency: UEFISCDI
Contract number: 77/2018
Project code: PN-III-P1-1.1-PD-2016-1562
Project acronym: NANO-BIO-INT
Project duration: 24 months (02.05.2018 – 30.04.2020)
Total funding: 250000 RON
Project members: Lect. Univ. Dr. Miruna-Silvia STAN (postdoctoral researcher); Prof. Univ. Dr. Marieta COSTACHE (mentor)
It is widely accepted that upon the nanoparticles (NPs) entrance to the biological fluid their surface will interact with the medium’s components providing a new biological identity for NPs. The interface between particles and cell surface lipid bilayer comprises a series of dynamic interactions which are mediated by the protein corona and it is of great importance to follow the progressive events triggered during exposure to NPs at different time points to obtain relevant analysis valuable for therapeutic drug delivery or other purposes. Therefore, this work envisages a complex characterization of NPs interaction with the three major classes of biomolecules in order to associate the changes in nano-bio-interface with the specific physicochemical properties of NPs and to obtain certain relationships very useful for further molecular modeling and simulation studies.
The objective of the present project is represented by the complex analysis over time of the interaction between nanoparticles with different characteristics (chemical structures, sizes, surfaces) and biomolecules (proteins, lipids and nucleic acids).
Expected results for this project
The expected results will include a deeper understanding of nanoparticles’ biocorona, of their behavior in biological systems, and effect on molecules’ structures, in order to design further strategies for preventing toxicity induced by nanoparticles with well-established properties.
Dissemination of results
– oral presentations and posters at national and international conferences
- Stan M.S., Nica I.C., Miu B., Dinischiotu A. “Are TiO2 nanoparticles genotoxic? Their influence on the cell cycle of human pulmonary fibroblasts”, Al XII-lea Congres National de Citometrie, 30-31 Mai, 2019, Bucuresti, Romania.
- Stan M.S., Mernea M., Cristian R.E., Mohammad I.J., Lambert C., Dinischiotu A. “Evaluation of titanium dioxide nanoparticles effect on phagocytic activity of granulocytes”, Al XII-lea Congres National de Citometrie, 30-31 Mai, 2019, Bucuresti, Romania.
- Stan M.S., Cristian R.E., Mohammad I.J., Mernea M., Sbarcea B.G., Trica B., Dinischiotu A. “Analyzing the interaction between different types of nanoparticles and serum albumin”, 21st Romanian International Conference on Chemistry and Chemical Engineering, September 4-7, 2019, Constanta-Mamaia, Romania.
- Nica I.C., Dinischiotu A. Stan M.S. “Genotoxic potential of TiO2 nanoparticles with enhanced photocatalytic properties investigated by Comet assay in human lung cells”. 13th International Comet Assay Workshop, 24-27 June, Pushchino, Moscow, Russia, p. 65 (ICAW 2019).
- Strugari A., Stan M.S. Nanoparticle intestinal transport characterization using in vitro co-culture models. Proceedings of 1st International Online-Conference on Nanomaterials (01.09-15.09.2018), DOI 10.3390/IOCN_2018-1-05480. (Poster)
– research articles published in ISI journal
- Stan MS, Mernea M, Cristian RE, Mohammad IJ, Sbarcea BG, Trica B, Dinischiotu A. Correlating the serum albumin corona of zinc oxide nanoparticles with their physico-chemical properties. Rom. Rep. Phys. 2020, 72(1):602. Impact Factor: 1.940, RIS: 0.702. • Cristian RE, Mohammad IJ, Mernea M, Sbarcea BG, Trica B, Stan MS, Dinischiotu A. Analyzing the interaction between two different types of nanoparticles and serum albumin. Materials 2019, 12(19), 3183. Impact Factor: 2.972, RIS: 1.405.
- Stan MS, Nica IC, Popa M, Chifiriuc MC, Iordache O, Dumitrescu I, Diamandescu L, Dinischiotu A. Reduced graphene oxide/TiO2 nanocomposites coating of cotton fabrics with antibacterial and self-cleaning properties. J. Ind. Text. 2019, 49(3):277-293. Impact Factor: 1.884, RIS: 2.373.
- Stan MS, Chirila L, Popescu A, Radulescu DM, Radulescu DE, Dinischiotu A. Essential oil microcapsules immobilized on textiles and certain induced effects. Materials 2019, 12(12), 2029. Impact Factor: 2.972, RIS: 1.405.
- Voicu SN, Balas M, Stan MS, Trică B, Serban AI, Stanca L, Hermenean A, Dinischiotu A. Amorphous silica nanoparticles obtained by laser ablation induce inflammatory response in human lung fibroblasts. Materials 2019, 12, 1026. Impact Factor: 2.972, RIS: 1.405.
- Strugari AFG, Stan MS, Gharbia S, Hermenean A, Dinischiotu A. Characterization of nanoparticle intestinal transport using an in vitro co-culture model. Nanomaterials 2019, 9(1), 5. Impact Factor: 4.034, RIS: 1.630.
- Stan MS, Badea MA, Pircalabioru GG, Chifiriuc MC, Diamandescu L, Dumitrescu I, Trica B, Lambert C, Dinischiotu A. Designing cotton fibers impregnated with photocatalytic graphene oxide/Fe, N-doped TiO2 particles as prospective industrial self-cleaning and biocompatible textiles. Mat. Sci. Eng. C 2019, 94:318-332. Impact Factor: 4.959, RIS: 1.231.
– book chapter
- Stan MS, Strugari AFG, Balas M, Nica IC. Biomedical applications of carbon nanotubes with improved properties. In: Fullerenes, Graphenes and Nanotubes: A Pharmaceutical Approach (ed. Grumezescu AM), Elsevier, Oxford, UK, 2018, p. 31-65. Print ISBN: 978-0-12-813691-1.
– review papers
- Nica IC., Stan M.S., Dinischiotu A. Current photocatalytic applications of nano-scaled titanium dioxide in the new era of “smart” technologies. Rev. Biol. Biomed. Sci. 2018; 1(2): 43-53. DOI: 10.31178/rbbs.2018.1.2.1.
- Stan MS., Badea MA., Strugari AFG. Carbon nanotubes: properties, applications and toxicity. Rev. Biol. Biomed. Sci. 2019; 2(1): 12-18.