On Tuesday, 6 June 2023, Yoshihiro Ohmiya, PhD, Prime Senior Researcher of AIST (The National Institute of Industrial Science and Technology – Japan), will present the conference “Eukaryotic cell engineering for immune response evaluation: illuminating cells for smart screening of bioactive compounds”.
The event will begin at 15:00 and will take place at the “Dimitrie Brandza” Botanical Garden (1-3 Portocalelor Alley, B administrative body building).
An immune response consists in multiple physiological reactions occurring within an organism for the purpose of keeping a body homeostasis against several exogenous factors. For the purposes of the evaluating bioactive compounds, bioluminescence reporter assay system is a powerful tool. Bioluminescence is a simple reaction that is triggered by the addition of luciferin solution, and the equipment for measuring light intensity is simple and convenient. So, luciferases are suitable reporter enzymes for the quantitative measurement of gene expression. The AIST team originally established the multi-coloured reporter assay using different colour beetle luciferases, which can evaluate the bioactivity of compounds. Until now, reporter cell lines using our system have been used for identifying several bioactive compounds from natural resources for modulating the immune response or anti-stress. In this lecture, I will talk about the principle of bioluminescence tools and how to use them for identifying novel bioactive compounds from natural resources based on clarifying their bioactive mechanism.
Yoshihiro Ohmiya earned his M.Sc. and Ph.D. degrees in Biochemistry and Material Science from the Gunma University (Japan). In 1990, Dr. Ohmiya pursue post-doctoral training at the Osaka Bioscience Institute. In 1996, Dr. Ohmiya was appointed to the faculty of the Shizuoka University as an Associate Professor in the Department of Education. The National Institute of Industrial Science and Technology (AIST) recruited him in 2001 to head its biodynamic research group and directed basic bioluminescent research program. He in 2006-2009 held a secondary appointment as a Professor in the Department of Photobiology in School of Medicine, Hokkaido University. In 2012-2018, he was the Director of Biomedical Research Institute (BRI) in AIST and directed basic and application research in biomedical field. From 2019, he has been the Prim Senior Researcher of BRI. He is a co-author of over 180 research articles in peer-reviewed journals, over 60 review articles/book chapters and over 40 patents. He served as Editor of luminescence the international Journal of Bioluminescence and Chemiluminescence “Luminescence” and the past-President of Internal Society of Bioluminescence and Chemiluminescence. He has been the Distinguished Professor of VISTEC (in Thailand) and Visiting Professor of the University of Bucharest, OIT (Osaka Institute of Technology) and Tottori University.