PED 127/2017

Project leader:

PhD. Valentina Mitran

Phone number: 021.318.15.75/int 104

Fax : 021.318.15.75/int 102

E-mail: valentinamitran@yahoo.com

Coordinating Institution:

University of Bucharest

Faculty of Biology, Departament of Biochemistry and Molecular Biology

www.dbbm.bio.unibuc.ro

Partner Institutions:

University POLITECHNICA of Bucharest

Contractual authority: UEFISCDI

Project code: PN-III-P2-2.1-PED-2016-0952

Project type and number: PED 127/2017

Project title: Biomimetic 3D-printed porous scaffolds for bone tissue engineering

Acronym: BiOGRAFTPRINT

Project start date: 03.01.2017

Project end date: 30.06.2018

Duration: 18 months

Budget: 600 000 lei

Summary:

The project BioGraftPrint approaches an experimental and demonstrative research seeking the development of novel strategies for improving bone healing by means of advanced materials, methods and technologies. In line with this, the project aims at developing novel Zr2+/Sr2+-doped hydroxyapatite/collagen/chitosan –synthetic bone grafts with well-defined and controllable architecture by means of a new generation technique, namely 3D printing, which ensures high precision and customized production. In view of implementing the demonstrator model, the project begins from a conceptual level, represented by the compositional and morphological design of the product, which will be materialized by initiation of the active process of research and development involving material synthesis and characterization, as well as preliminary in vitro and in vivo laboratory studies. The successful accomplishment of relevant activities will be assured by a close synergistic cooperation between project partners with complementary expertise in the fields of bioengineering, material science, biology and medicine. Their previously cooperation in many national/international projects concerning biomaterials development represent a starting point for the further project implementation. Thus, to satisfy the project objectives, UPB will be responsible with the synthesis and characterization of the new graft materials, while UB will be involved in the in vitro biological evaluation and in vivo testing of the novel 3D-printed materials. Original contribution of each partner will be disseminated in order to generate novel knowledge regarding particular relations between material composition/physical/mechanical characteristics and biocompatibility/host tissue responses. By achieving these tasks, the current project will represent a step further towards the development of new bio-inspired materials which are expected to increase life expectancy of patients with bone related traumas.

The project objective is to develop novel Zr2+/Sr2+ doped hydroxyapatite /collagen/chitosan -synthetic bone grafts with well-defined and controllable architecture by means of 3D printing and, also, to investigate there in vitro and in vivo behavior.

The specific objectives of this project are:

Establishing the technology of substituting Ca2 +from HA with Sr2 + or Zn 2+

Development and demonstration of 3D printing technology

Synthesis of 3D interconnected scaffolds based on Sr2+/Zn2+– doped COLL/HA/CS

Evaluation of osteoblasts and macrophages response to the novel 3D-printed substrates

Obtaining of the Demonstrator Product – implants obtained by 3D printing

Assessment of the in vivo integration potential of the new developed implants

Expected results of the project are:

New knowledge regarding the development of the 3D printing technology

Obtaining the demonstrator product -3D printed composite scaffolds whose performance will be demonstrated by advanced biological in vitro and in vivo assays

New knowledge regarding development of 3D printed scaffolds and cell-material interactions, which will be disseminated through the project web site, ISI scientific papers and communications to scientific national and international reunions

Participation at international/national scientific reunions:

1) V. Mitran, P.Neacsu, R.N. Ion, D. Gudovan, D. Ficai, R. Trusca, A. Ficai, A. Cimpean Osteoblasts behavior on 3D printed collagen/hydroxyapatite scaffolds designed for bone tissue regeneration, International Bone-Tissue-Engineering Congress (Bone-Tec), 12-14 October 2017, Munich, Germania, poster

2) D. Gudovan, I. L. Ardelean, D. Ficai, I. A. Gudovan, P. Balaure, R. D. Trusca, M. G. Albu-Kaya, V. Mitran, A. Cimpean, A. Ficai, E. Andronescu, 3D-printed collagen-hydroxyapatite scaffolds modified with essential oils with anti-inflammatory/antibiotic properties, Conferinta internațională:20th Romanian International Conference on chemistry and chemical engineering, Romania/ Poiana Brasov, 6- 9 september 2017, poster

3) R. Tiplea, M. G. Albu-Kaya, M. Sonmez, M. Ghica, D. Ficai, A. Ficai, I. L. Ardelean, D. Gudovan, E. Andronescu, Collagen/Hydroxyapatite composite supports for bone tissue engineering, 8th International Conference on Nanotechnology: Fundamentals and Applications (ICNFA’17), Roma, Italia, 5-9 june 2017, poster, award for the best presentation

4) D. Ficai, D. Gudovan, M. Sonmez, A. Ficai, R. D. Trusca, E. Andronescu, 3D Printed Collagen/Hydroxyapatite scaffolds loaded with Chamomile Essential Oils, 19th International Conference on Nanotechnology and Nanomedicine (ICNN 2017), Praga, Cehia, 8-11 july 2017, oral prezentation

5) D. Ficai, A. Ficai, D. Gudovan, I. A. Gudovan, I. Ardelean, R. Trusca, E. Andronescu, V. Mitran, A. Cimpean, Collagen/Hydroxyapatite compositions doped with transitional metals for bone tissue engineering applications, 19th International Conference on Environmental, Biomedical and Biotechnology (ICEBB 2017), Venetia, Italia, 12-15 november 2017 (e-poster)

6) V. Mitran, D. Ficai, A. Ficai, R. Ion, M. G. Necula, D. Gudovan, R. Trusca, E. Andronescu, A. Cimpean, In vitro preliminary assessment of cellular response to collagen/hydroxyapatite scaffolds designed for bone regeneration, The 36th Annual Scientific Session of the Romanian Society for Cell Biology and the 10thNational Congress with International Participation, june 6-9, Craiova, Romania, Bulletion of Romanian Society for Cell Biology No.46-June 2018, p 100, ISSN:1584-5532; ISSN-L:1584-5532, poster

7) A. Ficai, M. G. Albu-Kaya, D. Ficai, V. Mitran, A. Cimpean, E. Andronescu, Materialele compozite polimer/ceramică și procedeu de obținere a acestora, the International Salon of Scientific Research, Innovation and Invention PRO INVENT, 16th edition, 21-23 march 2018, Multipourpose Hall, Cluj-Napoca

Awards:

–Diploma of Excellence and Gold Medal from the State University of Medicine and Pharmacy “Nicolae Testemitanu” Chisinau, Moldova

– Diploma of Excellence from The Justin Capra Association (Romania)

– Gold Medal of the Jury of the International Salon of Scientific Research, Innovation and Invention PRO INVENT

8) A. Ficai, M. G. Albu-Kaya, D. Ficai, V. Mitran, A. Cimpean, E. Andronescu, Materialele compozite polimer/ceramică și procedeu de obținere a acestora, European Exhibition of Creativity and Innovation – 10th Edition of EUROINVENT-, Iasi, Romania, 17-19 may 2018

9) A. Ficai, M. G. Albu-Kaya, D. Ficai, V. Mitran, A. Cimpean, E. Andronescu, Materialele compozite polimer/ceramică și procedeu de obținere a acestora, The 22nd International Exhibition of Inventics “INVENTICA 2018”, 27 – 29 june 2018, Iaşi, Romania, Organized by Tehnical University “Gheorghe Asachi” from Iași.

ISI ARTICLES

2. L. Ardelean, D. Gudovan, D. Ficai, A. Ficai, E. Andronescu, M. G. Albu-Kaya; P. Neacșu, R. N. Ion, A. Cimpean, V. Mitran; Collagen/hydroxyapatite bone grafts manufactured by homogeneous/ heterogeneous 3D printing; under review la Materials Letters IF 2.572

PATENTS

7. Ficai, M. G. Albu-Kaya, D. Ficai, V. Mitran, A. Cimpean, E. Andronescu, Materialele compozite polimer/ceramică și procedeu de obținere a acestora, patent application No. A 01045/07.12.2017 – OSIM.