Biomimetic peptide scaffolds affording tunable biosensor interfaces

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AbstractBiosensors capable of sensing and responding to small molecules have wide-ranging applications, but despite such broad utility, no single strategy for the construction of biosensors has proven sufficiently generalizable to gain widespread use. The project aim is to design novel biomimetic peptide scaffolds generating biosensor interfaces, which can be tuned as common biorecognition interface for different transducers. The modular peptide-based sensors proposed will exploit the conductive and self-assembling properties of short helical peptides along with their ability to easily form amide, thioether and ester bonds in the presence of cross-linking reagents. This approach makes it possible to produce a wide variety of biosensors for different small molecules detection with application in clinical laboratory, environmental and food quality monitoring.

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Contract: code PN-III-P4-ID-PCE-2016-0288, no. 20 from 12/07/2017

Acronym: PEPSENS

Contracting authority: UEFISCDI

Duration of the project: 30 months (2017-2019)

Project Director:
Prof. dr. Camelia Bala
University of Bucharest
Department of Analytical Chemistry
Tel./Fax: +4021 410 48 88
Email: camelia.bala@chimie.unibuc.ro

Team members:
Drd. Madalin Danila
ACS dr. Lucian Gabriel Zamfir
ACS dr. Oana Maria Dumitrascu
Lector dr. Mihaela Elena Puiu
Conf. Dr. Lucian Rotariu

Objectives:
– Developing peptide self-assembled monolayers,
– Grafting the low molecular weight compounds onto peptide assembled monolayers on gold,
– Characterization and optimization of biosensors response.

Expected results:
Biosensors for application in clinical laboratory, environmental and food quality monitoring.

Dissemination:

G. M. Danila, M. Puiu, L. G. Zamfir, C. Bala, Analytical Chemistry, 2019, https://doi.org/10.1021/acs.analchem.9b03845

M. Puiu, C. Bala, Current Opinion in Electrochemistry, 12, 2018, 13-20.

M. Puiu, C. Bala, Peptide-based electrochemical biosensors, in Electrochemical Biosensors, 2019, 277-306, Elsevier Inc. ISBN 978-0-12-816491-4

Building switchable peptide-architectures on gold/composite surfaces: New perspectives in electrochemical bioassays, M. Puiu, C. Bala, Current Opinion in Electrochemistry, 12, 2018, 13-20.

Peptide-based electrochemical biosensors, M. Puiu, C. Bala, in Electrochemical Biosensor, Ed. Ali. A. Ensafi, Elsevier Inc., 2019, 277-306, ISBN: 978-0-12-816491-4

Puiu, L.-G. Zamfir, V. Buiculescu, A. Baracu, C. Mitrea, C. Bala, Sensors, 18(10), 2018, 3541

Braik, L.-G. Zamfir, L. Rotariu, C. Curutiu, M.C. Chifiriuc, M.B. Ali, C. Bala, Sensors and Actuators B: Chemical 273, 2018, 298-304

Peptide-based biosensors: From self-assembled interfaces to molecular probes in electrochemical assays, M. Puiu, C. Bala, Bioelectrochemistry, 120, 2018, 66-75

Molecular Recognition: Versatility and Challenges in the Design of Nanobiosensors for Food Security, M. Puiu, C. Bala, in Trends in Food Safety and Protection, Edited by Ravishankar Rai V. and Jamuna A. Bai, Taylor & Francis Ltd., 2017, 51-82

G.M. Dănilă, M. Puiu, L. G. Zamfir, C. Bala, Dezvoltarea unei metode imunoanalitice pentru detecția analogilor grelinei, Conferința națională a școlilor doctorale din Consorțiul Universitaria, Ediția I, Iași, 1-3 November 2018 (oral presentation)

Bala, M. G. Dănila, L. G. Zamfir, M. Puiu, Peptide biosensor for small ligands detection, 33° Latino-American congress of Chemistry, CLAQ and the X Congress of Chemical Science, Technology and Innovation, QUIMICUBA’2018, Havana, Cuba, 9 – 12 October 2018 (oral presentation)

Bala, The 8th International Workshop on Surface Modification for Chemical and Biochemical Sensing Programme, Żelechów, Poland, 3 – 7 November 2017 (Key note)

G.M. Dănilă, L. G. Zamfir, M. Puiu, C. Bala, The 8th International Workshop on Surface Modification for Chemical and Biochemical Sensing Programme, Żelechów, Poland, 3 – 7 November 2017 (poster)

Bala, XXIV International Symposium on Bioelectrochemistry and Bioenergetics, 3-7 July, 2017, Lyon, France (invited lecture)

Rotariu, L-G. Zamfir, S. Teka, C. Bala, 17th International conference on Electroanalysis (ESEAC 2018), Rhodes, Greece, 3-7 June 2018 (oral presentation)

Puiu, L. G. Zamfir, M. G. Dănilă, C. Bala, Redox – labelled peptide monolayers as universal supports for small ligands in electrochemical ligand-binding assays, 17th International conference on Electroanalysis (ESEAC 2018), Rhodes, Greece, 3-7 June 2018 (oral presentation)

Results 2017 Results 2018  Results2019