Habilitation thesis: Developing strategies for in vitro biocompatibility assessment of novel biomaterials   Habilitation Abstract_Cimpean

Books, Monographies and Courses

1. Cimpean A, Ion R, Ciofrangeanu C, Mitran V, Galateanu B, Iordachescu D, Practical guide for in vitro evaluation of dental implant biocompatibility, 2008, Editura Ars Docendi, ISBN: 978-973-558-364-4 (in romanian language).
2. Gavrila M., Cimpean A., Method of isolation and culture of human mesenchymal stem cells, 2007, Editura Curtea Veche, ISBN 978-973-9467-92-6 (in romanian language).
3. Cimpean A, 2006, Animal Cell Cultures, Editura Ars Docendi, ISBN-10 973-558-274-0; ISBN-13 978-973-558-274-6 (in romanian language).
4. Iordachescu D., Cimpean A., Stan C., 2004, Extracellular matrix remodeling. Applications in tissue engineering, Editura Pro-Transilvania, ISBN 973-715-024-4 (in romanian language).

Book chapters

1. Cimpean, A., Ion, R., Gordin D.-M., Neacsu, P., Mitran, V., Gloriant, T., 2013, Biomaterials as modulators of macrophage inflammatory response, Biomaterials (vol. 11) in: N.K. Navani, S. Sinha (Eds.), J.N. Govil (Executive Ed.), Nanotechnology series, Studium Press LLC (USA), p. 139-176, ISBN: 1-62699-000-X.
2. Dimonie D, Petrache M, Doncea S, Gabor R, Vasile E, Cimpean A, Fierascu RC, Cristian N, Dinescu S, Trandafir I, Anton L, Fierascu I, Galateanu B. 2013. New approaches to develop 3D layer shaped scaffolds for soft tissue engineering, in: Advanced biocompatible structures for prospective bioengineering: Concepts and strategies, The Publishing House of the Romanian Academy, p. 74-124, ISBN: 978-973-27-2317-3.

Papers published in ISI indexed journals (selective)

• Neacsu P, Staras AI, Voicu SI, Ionascu I, Soare T, Uzun S, Cojocaru VD, Pandele AM, Croitoru SM, Miculescu F, Cotrut CM, Dan I, Cimpean A. Characterization and In vitro and In vivo Assessment of a Novel Cellulose Acetate-coated Mg-based Alloy for Orthopaedic Applications, Materials, 2017, 10 (7), 686 (2017) http://www.mdpi.com/1996-1944/10/7/686/htm
• Voicu SI, Condruz RM, Mitran V, Cimpean A, Miculescu F, Andronescu C, Miculescu M, Thakur VK. Sericin Covalent Immobilization onto Cellulose Acetate Membrane for Potential Osseointegration Applications. ACS Sustainable Chem Eng, 4, 1765-1774 (2016).http://pubs.acs.org/doi/abs/10.1021/acssuschemeng.5b01756
• Ion R, Luculescu C, Cimpean A, Marx P, Gordin D-M, Gloriant T. Nitride coating enhances endothelialization on biomedical NiTi shape memory alloy. Mater Sci Eng C, 62, 686–691 (2016). http://www.sciencedirect.com/science/article/pii/S0928493116301217
• Castany P, Gordin D-M, Drob SI, Vasilescu C, Mitran V, Cimpean A, Gloriant T. Deformation mechanisms and biocompatibility of the superelastic Ti–23Nb–0.7Ta–2Zr–0.5N alloy. Shap Mem Superelasticity, 2, 18-28 (2016). http://link.springer.com/article/10.1007/s40830-016-0057-0
• Rusen L, Neacsu P, Cimpean A, Ion V, Brajnicova S, Dumitrescu LN, Banita J, Dinca V, Dinescu M. In vitro evaluation of poly(ethyleneglycol)-block-poly(e-caprolactone) methyl ether copolymer coating effects on cells adhesion and proliferation. Appl Surf Sci, 374, 23-30 (2016).http://www.sciencedirect.com/science/article/pii/S0169433215020383
• Ion R, Stoian AB, Dumitriu C, Grigorescu S, Mazare A, Cimpean A, Demetrescu I, Schmuki P. Nanochannels formed on TiZr alloy improve biological response. Acta Biomater, 24, 270-377 (2015). http://www.ncbi.nlm.nih.gov/pubmed/26092351
• Neacsu P, Mazare A, Schmuki P, Cimpean A. Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-kB pathways. Int J Nanomedicine, 10, 6455-6476 (2015).        http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608594/
• Mitran V, Vasilescu C, Drob SI, Osiceanu P, Calderon Moreno JM, Tabirca MC, Gordin DM, Gloriant T, Cimpean A. Biological behaviour and enhanced anticorrosive performance of the nitrided superelastic Ti-23Nb-0.7Ta-2Zr-0.5N alloy. BioMed Res Int, 125, article ID  261802 (2015).http://sci-hub.cc/10.1155/2015/261802
• Drob SI, Vasilescu C, Calderon Moreno JM, Osiceanu P, Neacsu P, Cimpean A, Gordin DM, Gloriant T. Multifunctional “gum metal” titanium-based alloy: Its long-term electrochemical behaviour and macrophage response. Int J Electrochem Sci, 10, 10738-10755 (2015). http://www.electrochemsci.org/papers/vol10/101210738.pdf
• Mitran V, Albu MG, Vasile E, Cimpean A, Costache M. Dose-related effects of sericin on preadipocyte behavior within collagen/sericin hybrid scaffolds. Prog Nat Sci Mater Internat, 25, 122-130 (2015). http://authors.elsevier.com/a/1R5SJ3Zr7s9kfE(free access)
• Neacsu P, Ion RN, Mitran V, Staras AI, Cimpean A. State of the art and recent patents on Mg-based biodegradable bone implants. Rec. Pat. Regen. Med., Vol. 4, Nr. 3, 168-188 (2015). DOI: 2174/2210296505666150205155811
• Ion R, Vizireanu S, Stancu CE, Luculescu C, Cimpean A, Dinescu Gh. Surface plasma functionalization influences macrophage behavior on carbon nanowalls, Mater Sci Eng C Mater Biol Appl, 48, 118-125 (2015). http://www.ncbi.nlm.nih.gov/pubmed/25579904
• Neacsu P, Gordin DM, Mitran V, Gloriant T, Costache M, Cimpean A. In vitro performance assessment of new beta Ti-Mo-Nb alloy compositions, Mater Sci Eng C Mater Biol Applic, 47, 105-113 (2015). http://www.ncbi.nlm.nih.gov/pubmed/25492178
• Neacsu P, Mazare A, Cimpean A, Park J, Costache M, Schmuki P, Demetrescu I. Reduced inflammatory activity of RAW 264.7 macrophages on titania nanotube modified Ti surface, Int J Biochem Cell Biol, 55, 187-195 (2014).http://www.ncbi.nlm.nih.gov/pubmed/25220343
• Cimpean A, Vasilescu E, Drob P, Cinca I, Vasilescu C, Anastasescu M, Mitran V, Drob SI. Enhancement of the electrochemical behaviour and biological performance of Ti–25Ta–5Zr alloy by thermo-mechanical processing, Mater Sci Eng C Mater Biol Applic, 38, 127-142 (2014). http://www.ncbi.nlm.nih.gov/pubmed/24656361
• Cimpean A, Recent advances and challenges in adipose tissue engineering: Adipose derived stem cell-based approaches, Curr Tiss Eng, 3, 7-24 (2014). http://benthamscience.com/journal/abstracts.php?journalID=cte&articleID=119438
• Ion R, Gordin DM, Mitran V, Osiceanu P, Dinescu S, Gloriant T, Cimpean A, In vitro biocompatibility performances of the novel superelastic beta-type Ti-23Nb-0.7Ta-2Zr-0.5N alloy, Mater Sci Eng C Mater Biol Applic, 35, 411-419 (2014). http://www.ncbi.nlm.nih.gov/pubmed/24411395
• Ion R, Vasilescu C, Drob P, Vasilescu E, Cimpean A, Drob I, Gordin DM, Gloriant T. Long-term corrosion performances and cytocompatibility of nitrided Ti and Ti–6Al–4V alloy in severe functional conditions, Mater Corros, 65, 593-604 (2014). http://onlinelibrary.wiley.com/doi/10.1002/maco.201206724/abstract
• Mindroiu M, Ion R, Pirvu C, Cimpean A, Surfactant-dependent macrophage response to polypyrrole-based coatings electrodeposited on Ti6Al7Nb alloy, Mater Sci Eng C Mater Biol Applic, 33, 3353-3361 (2013).
  http://www.sciencedirect.com/science/article/pii/S0928493113002300
• Andronescu E, Ficai A, Albu M, Mitran V, Sonmez M, Ficai D, Ion R, Cimpean A. Collagen-hydroxyapatite/Cisplatin Drug Delivery Systems for Locoregional Treatment of Bone Cancer, Technol Cancer Res Treat, 12, 275-284 (2013). http://www.ncbi.nlm.nih.gov/pubmed/23547973
• Gordin DM, Gloriant T, Chane-Pane V, Busardo D, Mitran V, Hoche D, Vasilescu C, Drob SI, Cimpean A. Surface characterization and biocompatibility of titanium alloys implanted with nitrogen by Hardion+ technology, J Mater Sci: Mater Med, 23, 2953-2966 (2012).
  http://www.ncbi.nlm.nih.gov/pubmed/22918550
• Cimpean A, Mitran V, Ciofrangeanu CM, Galateanu B, Bertrand E, Gordin D-M, Iordachescu D, Gloriant T. Osteoblast cell behavior on the new beta-type Ti–25Ta–25Nb alloy, Mater Sci Eng C Mater Biol Applic, 32, 1554–1563 (2012).
  http://www.sciencedirect.com/science/article/pii/S0928493112001610
• Galateanu B., Dimonie D., Vasile E., Nae S., Cimpean A., Costache M., Layer-shaped Alginate Hydrogels Enhance the Biological Performance of Human Adipose-Derived Stem Cells, BMC Biotechnol 12, 35, http://www.biomedcentral.com/1472-6750/12/35 (2012). http://www.biomedcentral.com/1472-6750/12/35
• Galateanu B., Dinescu S., Cimpean A, Dinischiotu A, Costache M., Modulation of adipogenic conditions for prospective use of hADSCs in adipose tissue engineering, Int J Mol Sci, 13, 15881-15900, doi:3390/ijms130x000x(2012).
  http://www.ncbi.nlm.nih.gov/pubmed/23443100
• Dinescu S., Galateanu B., Albu M., Cimpean A., Dinischiotu A., Costache M., Sericin Enhances the Bioperformance of Collagen-Based Matrices Preseeded with Human-Adipose Derived Stem Cells (hADSCs), Int J Mol Sci, 14, 1870-1889; doi:3390/ijms14011870(2013).
  http://www.mdpi.com/1422-0067/14/1/1870
• Vasilescu C, Calderon Moreno JM, Cimpean A, Cojocaru D, Cinca I, Andronescu E, Grigore F, Galateanu B, Drob SI, Synthesis, mechanical and structural properties and biological activity of some nanostructured bone scaffolds, Digest J Nanomat Biostr, 6, 523-534 (2011). http://connection.ebscohost.com/c/articles/74592904/synthesis-mechanical-structural-properties-biological-activity-some-nanostructured-bone-scaffolds
• Cimpean A, Popescu S, Ciofrangeanu CM, Gleizes AN. Effects of LP-MOCVD prepared TiO2 thin films on the in vitro behavior of gingival fibroblasts, Mat Chem Phys, 125, 485-492 (2011).
  http://www.sciencedirect.com/science/article/pii/S025405841000859X
• Raducanu D, Cimpean A, Vasilescu E, Cojocaru VD, Cinca I, Drob P, Ivanescu S. Corrosion behaviour and biocompatibility of a new dental noble AuPdAgTi alloy, Mater Corros, 61, 775-782 (2010). http://onlinelibrary.wiley.com/doi/1002/maco.201005636/abstract
• Scorei RI, Ciofrangeanu C, Ion R, Cimpean A, Galateanu B, Mitran V, Iordachescu D. In Vitro Effects of Calcium Fructoborate upon Production of Inflammatory Mediators by LPS-stimulated RAW 264.7 Macrophages, Biol Trace Elem Res,135, 334-344 (2010).
  http://www.ncbi.nlm.nih.gov/pubmed/19669712
• Scorei R, Ciubar R, Ciofrangeanu CM, Mitran V, Cimpean A, Iordachescu D. Comparative effects of boric acid and calcium fructoborate on breast cancer cells, Biol Trace Elem Res, 122, 197-205 (2008).
  http://www.ncbi.nlm.nih.gov/pubmed/18176783
• Popa MV, Demetrescu I, Iordachescu D, Cimpean A, Vasilescu E, Drob P, Vasilescu C, Istratescu M, The relation between electrochemical tests and in vitro evaluation of titanium alloy biocompatibility, Mater Corros, 58, 667-675 (2007).
  http://onlinelibrary.wiley.com/doi/1002/maco.200704053/abstract
• Ciubar R, Ciofrangeanu C, Mitran V, Cimpean A, Stanescu A, Iordachescu D, The effect of lead ions on human erythrocytes in vitro, Rev Chim – Bucharest, 9, 895-808 (2007).
  http://revistadechimie.ro/pdf/CIUBAR%20R..pdf
• Scorei R, Ciubar R, Iancu C, Mitran V, Cimpean A, Iordachescu D, In vitro effects of calcium fructoborate on fMLP-stimulated human neutrophils granulocytes, Biol Trace Elem Res, 118, 27-37 (2007).
  http://link.springer.com/article/10.1007%2Fs12011-007-0019-7#page-1
• Ciubar R, Mitran V, Cimpean A, Iordachescu D, In vitro effects of nickel on human embrionary lung fibroblasts, Rev Roum Chim, 51, 199-203, 2006.
  http://revroum.getion.ro/wp-content/uploads/2006/RRC_3_2006/Art.%2006.pdf
• Cimpean A, Stefan C, Gijsbers R, Stalmans W, Bollen M, Substrate-specifying determinants of the nucleotide pyrophosphatases/phosphodiesterases NPP1 and NPP2, Biochem J, 381, 71-77 (2004).
  http://www.ncbi.nlm.nih.gov/pubmed/15096095

Lectures

A.Bachelor’s cycle

1. Clinical Biochemistry

This course (20 h) is addressed to the students from Bachelor’s cycle (3rd year of study) – section of Biochemistry and approaches the next topics: 

1. Introduction in clinical pathology and applications of the medical biochemistry in the laboratory diagnostic.
2. Blood: Plasma and specialized cells; Anemias: macrocitic anemia (megaloblastic anemia, pernicious anemia), genetic hemolytic anemias: ereditary spherocytosis, ereditary elliptocytosis, deficiency in glucose-6-P-dehydrogenase, hemoglobinopathies (sickle cell anemia, thalassemias); Diseases associated to disturbances of iron metabolism (iron deficiency anemia, hemosiderosis, hemochromatosis).
3. Hepatic and biliary disorders: Biochemical tests of liver function; Hepatobiliary diseases: jaundice (hemolytic, hepatocellular and obstructive icterus), inherited disorders of bilirubin metabolism; Hepatitis; Hepatic fibrosis/cirrhosis.
4. Pancreas disorders: Acute and chronic pancreatitis,  cystic fibrosis (mucoviscidosis) pancreatic
5. Renal patophysiology: Renal functions with clinical significance; Abnormal constituents of urine; Renal diseases: glomerular diseases (glomerulonephritis, nephrotic syndrome), tubular disorders of the kidney (renal tubular acidosis), urinary tract infections, urinary tract obstruction, kidney failure, diabetic nephropathy, renovascular hypertension.
6. Heart pathophysiology: Miocardic enzymes with clinical significance; Heart disorders: ischemic heart disease, myocardial infarction, heart failure, cardiomiopathy, heart arrhythmias, valvular heart diseases.
7. Pathophysiology of artery diseases: Regulation of the vascular tonus, atherosclerosis, arterial hypertensions, arterial hypotension.

B. Master’s cycle

1. Teoretical and practical aspects of animal cell cultures

This course addresses to the students from Master’s cycle (1st year of study) – Master of Biochemistry and Molecular Biology. Specific topics include:

• History, objectives, advantages and disadvantages of animal cell cultures.
• Cell culture systems: tissue, organ and cell cultures; organotypic and histotypic cultures; mass and clonal cell cultures; adherent cell culture vs. suspension cell culture; primary cell cultures, finite cell lines and continuous cell lines.
• Cell culture media and cell culture conditions.
• Growth cycle and cell proliferation in culture.
• Cell syncronization: chemical and physical methods.
• Cell immortalization: Spontaneous immortalization; Methods to obtain immortal cells (DNA tumor viruses, telomere maintainance, ALT-Alternative Lenghtening of Telomeres, expression of adenoviral and retroviral oncogenes, carcinogens and irradiation); Practical aspects of the immortalization of primary cells.
• Cell transfection: Types of transfection; Cell culture conditions to transfect cells in culture; Methods for cell transfection; Reporter gene systems.
• Cell transformation: Genomic instability; Cell immortality; Aberrant cell growth control; Tumorigenicity of transformed cells.
• Cellular senescence: Types of cellular senescence and characteristics of senescent cells; The signals and molecular pathways activating the cellular senescence; Mechanisms of induction of cellular senescence.
• Cell quiescence, cell differentiation  and cell de-differentiation.
• Flow cytometry;
• Application of cell cultures in obtaining monoclonal antibodies.

2.Introduction in tissue engineering

This course (14 h) addresses to the students from Master’s cycle (2nd year of study) – Master of Biochemistry and Molecular Biology. Specific topics include:

• Introduction to tissue engineering;
• Basic principles of tissue engineering in vitro si in vivo: cellular component (stem cells, differentiated cell), scaffolds (polymeric matrices), signaling molecules.
• Biocompatibility: in vitro tests to evaluate the biomaterial biocompatibility (tests included in ISO 10993-5 standards; specific tests to assess the cell-biomaterial interactions); In vivo tests to evaluate the biomaterial biocompatibility (tests of sensitivity, intracutaneous reactivity, systemic toxicity, genotoxicity, carcinogenicity, etc.).
• Applications of tissue engineering in regenerative medicine: Skin tissue engineering, Bone tissue regeneration and tissue engineering; Cartilage reconstruction by tissue engineering; Vascular tissue engineering; Liver tissue engineering.

Papers published in Journals with referees (1998-2007)

• Cimpean A, Caloianu M, Zarnescu O, Moldovan L, Efimov N, Ranga O, Structural, ultrastructural and biochemical aspects of the cartilaginous extracellular matrix degradation in human knee osteoarthritic lesions, J Med Biochem, 2, 4, 313-322 (1998). Articol
• Cimpean A, Caloianu M, Zarnescu O, Bojinca M, Synovial Membrane Pathology and its Implication in Human Osteoarthritis, J Med Biochem, 3, 4, 379-387 (1999). Articol
• Cimpean A, Caloianu M, Zarnescu O, Bojinca M, Synovial fluid alterations in human rheumatoid arthritis, J Med Biochem, 3, 1, 79-86 (1999). Articol
• Cimpean A, Caloianu M, Alexandru D, Efimov N, Buzgariu W, Effect of the interleukin-1b on gelatinolytic activity and cell morphology of human osteoarthritic chondrocytes in culture, J Med Biochem, 4, 2, 113-129 (2000). Articol
• Cimpean A, Zarnescu O, Caloianu M, Moldovan L, Efimov N, Glycosaminoglycan synthesis by human osteoarthritic chondrocytes cultured in alginate beads, Roum Biotechnol Lett, 5, 2, 127-135 (2000). Articol
• Cimpean A, Moldovan L, Flamanzeanu M, Nicolae C, In vitro polymolecular aggregation and ultrastructural characterization of the osteoarthritic cartilage proteoglycans, Rev Roum Biol – Biol Anim, Tome 46, 1-2, 85-92 (2001).Articol
• Moldovan L, Zarnescu O, Oancea A, Cimpean A, Constantinescu L, Corneal collagen interaction with proteoglycans and glycosaminoglycans, Roum Biotechnol Lett, 5, 2, 137-145 (2002). Articol
• Buzgariu W, Zarnescu O, Caloianu M, Cimpean A, Titescu G, Stefanescu I, Effects of heavy water on ultrastructural and functional status of HEP2 and CHO cells lysosomes, Revue Roum Biol- Biol Anim, Tome 47, 1-2, 87-96 (2002). Articol
• Cimpean A, Caloianu M, Zarnescu O, Batrana D, Cartilage alterations in human osteoarthritis, Rom J Biol Sci, IV, 1-4, 27-34 (2002). Articol
• Cimpean A, Zarnescu O, Moldovan L, Nicolae C, Flamanzeanu M, In vitro evaluation of biosynthetic activity of the human osteoarthritic chondrocytes embedded in collagen gel, Roum Biotechnol Lett, 7, 6, 987-994 (2002). Articol
• Moldovan L, Cimpean A, Flamanzeanu M, Nicolae C, Dumitrescu D, Matrix metalloproteinases involved in injured corneal tissue remodelling, Roum Biotechnol Lett, 7, 1, 14-27 (2002). Articol
• Homeghiu CL, Bucata G, Bonoiu A, Stan C, Mitran V, Cimpean A, Iordachescu D, In vitro effects of transforming growth factor b1 in a cadmium chloride model of lung injury, Rom J Biophys, 14, 21-28 (2004). Articol
• Stan C, Cimpean A, Iordachescu D, Cellular and molecular changes of psoriatic keratinocytes in response to UVA in vitrotreatment, Rom J Biophys, 14, 1-4, 1-12 (2004). Articol
• Cimpean A, Endogenous proteases involved in human osteoarthritic cartilage degradation, Analele Universitatii Bucuresti, LIII, 15-26 (2004). Articol
• Cimpean A, Zarnescu O, An ultrastructural study of cartilage alterations in human osteoarthritis, Revue Roum Biol- Biol Anim, Tome 50, 1-2, 99-105 (2005). Articol
• Cimpean A, Zarnescu O, Stan C, Diaconeasa A, Nae S, Iordachescu D, Studii comparative privind exprimarea moleculelor Bcl-2 si Bax de catre keratinocitele normale si psoriazice, Analele Societatii Nationale de Biologie Celulara, IX, Nr. 2, 257-261 (2005). Articol
• Neagos D, Mitran V, Chiracu G, Cimpean A, Cocos R, Iordachescu D, Effects of insulin, retinoic acid and hydrocortisone on in vitro dermal wound healing model, Rom J Biophys, 16, 93-102 (2006). Articol
• Ciubar R, Mitran V, Bonoiu A, Cimpean A, Iordachescu D, The effect of nickel on the fibroblast mediated collagen matrix contraction, Rom J Biophys, 16, 125-133 (2006). Articol
• Neagos D, Mitran V, Chiracu G, Ciubar R, Iancu C, Stan C, Cimpean A, Iordachescu D, Skin wound healing in a free floating fibroblast populated collagen latice model, Rom J Biophys, 16, 157-168 (2006). Articol
• Stan C, Cimpean A, Zarnescu O, Iordachescu D, Pattern of keratin distribution in cultured psoriatic keratinocytes, Dermato.ro., 15, 36-39, 2007. Article

Projects

PCE 188 Project

PN-II-ID-JRP-2012

PCCA 195-2014

PED-149-2017

PCE-55-2017

Manunet-31-2017