Type 2 Diabetes: a multi-OMIc view on host NAPH oxidases (NOx) and gut microbiota

Abstract: Development of type 2 diabetes(T2D) is a very complex, gradual and heterogeneous process. Importantly, diabetic individuals exhibit differences in terms of gut microbiome signatures, metabolic profiles and host markers compared to healthy individuals. Even though the field of microbiota analysis has emerged in the last few years and many reports associate microbial changes to various ailments, integrative studies that target the host-microbe interactions in diabetes are still in their infancy. To gain a better understanding of the biological processes linked to T2D, we will perform a detailed longitudinal multi-omic analysis of diabetic patients  compared to healthy individuals targeting the  microbiome, metabolome and resistome profiles. Altogether, these analyses will provide a rich source of data to characterize the host-microbe interactions associated with T2D.

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Contract no.: PD 224

Project code: PN-III-P1-1.1-PD-2019-0499

Acronim: DOMINO

Project director:
Dr. Grațiela Grădișteanu

Objective: This study aims to correlate and integrate for the first time microbiome, resistome, metabolome and NOX profiles in type 2 diabetes patients. This innovative approach will broaden our understanding regarding the molecular features of a disease with epidemic proportions.

Expected results: The DOMINO study has the potential to significantly influence the scientific field. We will implement for the first time in Romania innovative experimental methods including intestinal microbiota next generation sequencing and metabolome analysis. During its implementation , the project will lead to a series of results including experimental data on microbiome, metabolome and NOX profiles of diabetic patients, integrative analysis algorithms, and  publication of at least 2 high impact ISI papers.